reprogramming-inspired

perspective on development

Karow Lab

 
Research

Uncovering the molecular logic of human neuron formation -

from direct reprogramming to cerebral organoids

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The vision of the Karow lab is to uncover mechanisms of human neuron formation and to identify new molecular targets not only for enhancing and navigating but also for correcting defective neurogenesis. We are integrating the strength of two complementary approaches to study the formation of human neurons, that is in forced and natural conditions. 

Through overexpression of neurogenic transcriptions factors we are forcing neurogenesis in non-neural brain-resident pericytes of the adult human cerebral cortex to obtain lineage conversion into induced neurons (iNs). Our recent work has revealed not only an unprecedented understanding of the intermediate states during the fate change of pericytes into iNs, but also provided new insights into the molecular underpinning of neuron subclass specification. Based on these findings, we now aim at using the iN model system to identify transcriptional programs driving neuronal subtype specification in natural conditions. This will provide not only new cues on the specification of distinct neuron classes, but might also provide new insights in the molecular basis for many neurodevelopmental disorders (including neuropsychiatric disorders) in which this fine-tuned balance of neuron specification is altered.

The tools to investigate this "natural" neurogenesis are centered around cerebral organoids, a model system allowing investigation of early aspects of human neurogenesis. Currently, we are studying the disease etiology of a monogenetic X-linked disorder caused by an intellectual disability-causing mutation.

If you want to know more about our research, please contact us. We are always interested in motivated, curious people with a background in biology, bioinformatics, biomaterials, and medicine. 

 
Team
Marisa Karow, Dr
Professor
Ben Lohrer, MSc
Graduate Student
Elisa Gabassi, MSc
Intern
Doris Jaegers
Lab Technician
Dandan Han, Dr
PostDoc
Alumni
 
  • Karow M*#, Camp GJ*, Falk S, Gerber T, Pataskar A, Gac-Santel M, Kageyama J, Brazovskaja A, Garding A, Fan Wengiang, Riedemann T, Casamassa A, Schichor C, Götz M, Tiwari VK, Treutlein B#, Berninger B#. Direct pericyte-to-neuron reprogramming via unfolding of a neural stem cell-like program. 2018. Nature Neuroscience. * co-first authors  # co-corresponding authors.

 

Pubmed     Nature Neuroscience          PMC          GEO          ENA         Altmetric

  • Gascón S, Murenu E, Masserdotti G, Ortega F, Russo GL, Petrik D, Deshpande A, Heinrich C, Karow M, Robertson SP, Schroeder T, Beckers J, Irmler M, Berndt C, Angeli JP, Conrad M, Berninger B, Götz M. Identification and Successful Negotiation of a Metabolic Checkpoint in Direct Neuronal Reprogramming. 2016. Cell Stem Cell.

 

Pubmed           Cell Stem Cell          Preview         PlumX

  • Karow M, Schichor C, Beckervordersandforth R, Berninger B. Lineage-Reprogramming of Pericyte-Derived Cells of the Adult Human Brain into Induced Neurons. 2014. Journal of Visualized Experiments.

 

Pubmed           JOVE          PMC        

  • Karow M, Sánchez R, Schichor C, Masserdotti G, Ortega F, Heinrich C, Gascón S, Khan M, Lie DC, Dellavalle A, Cossu G, Goldbrunner R, Götz M, Berninger B.Reprogramming of pericyte-derived cells of the adult human brain into induced neuronal cells. 2012. Cell Stem Cell.

 

Pubmed           Cell Stem Cell          PlumX

Selected publications
  • Lan F, Liu J, Narsinh KH, Hu S, Han L, Lee AS, Karow M, Nguyen KH, Nag D, Calos MP, Robbins RC, Wu, JC. Safe Genetic Modification of Cardiac Stem Cells Using a Site-Specific Integration Technique. 2012. Circulation.

 

Pubmed           Circulation          PMC

 

Pubmed           Stem Cells          PMC

  • December 2019 Dandan joins the lab

  • October 2019 Elisa defended her MSc thesis

  • September 2019 Marisa is appointed as a professor at the Institute of Biochemistry in the Medical Faculty at the FAU Erlangen

  • July 2019 DFG project is funded

  • June 2019 Ben receives Boehringer Ingelheim travel award for the  EMBO course on developmental neurobiology

  • April 2019 Elisa joins the lab with an Erasmus fellowship

  • February 2019 ForInter project is funded

  • July 2018 Heiko visited the lab for a very fruitful collaboration

  • July 2018 Ben is accepted in the IMPRS-TP graduate school

  • June 2018 Our work is published in Nature Neuroscience. Great collaboration with Gray Camp and Barbara Treutlein

News
 
 
Lab life
 
Contact

Prof. Dr. Marisa Karow

Institute of Biochemistry

Room 02.021

Fahrstrasse 17

91054 Erlangen

​​

Tel:  +49 9131 85-24636

Fax: +49 9131 85-22484

marisa.karow@fau.de

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